For elucidating


01-Jul-2017 10:11

One strategy to account for this is to increase the length of the DNA sequence targeted, thus decreasing the probability of an unpredictable binding occurring randomly.A single zinc finger recognizes a 3-bp DNA sub-site, which may occur multiple times in a large genome.Although there is no simple, general code for zinc finger protein–DNA recognition, selection strategies have been developed that allow these proteins to be designed to target almost any desired site on double-stranded DNA.The Cys2His2 zinc finger proteins, and more often, Zif-268, offers a stable and versatile framework for the design of such proteins [].However, the absence of a standard rubric for predicting putative binding efficiency still plagues most approaches in targeted genome editing [].Most prediction methods reported in the literature focus either only on affinity or on specificity, almost completely ignoring the other aspect.

Moreover, the binding affinity of the individual zinc fingers was found to increase in the order Finger 1 The field of targeted genome engineering is still incipient, and, there is a compelling need to develop tools which can meet the ever growing requirements of the field: designing DNA templates of our choice, construction and manipulation of DNA sequences, and tools for the implementation, testing and debugging of genome editing experiments.Dissociation constants, typically measured by gel shift experiments, are reported in studies involving zinc finger protein–DNA complexes and offer a rough standard for the comparison of results.Another measure of specificity includes the comparison between the binding constant at the desired ZFP target site to that of the binding constant at various “mutant” sites wrt DNA.Such experiments typically show a two to ten-fold increase in the K value for each single base-pair change in the binding site, with mutations near the centre of the binding site usually having larger effects than mutations near the periphery.

These side directed mutational studies may aid computational analysis of the binding affinity altered by mutations of choice in the protein template.The rationale to study about zinc finger domain and its interaction with the DNA stems from the need to expatiate on the mechanisms by which the binding of transcription activators and repressors to the genome regulates the expression repertoire of all genes in a cell, hence focussing on its enormous scope in genome engineering.To exploit zinc finger proteins for genome manipulation, molecular and structural insights at the binding interface of zinc fingers and corresponding DNA targets are mandatory.The importance of designing ZFPs which bind to target DNA sequences is further aided by the developments in chemical methods for protein synthesis that enables the preparation of zinc finger proteins containing amino acids that do not usually occur in these proteins.



Oct 27, 2015. Development of Aortic Valve Disease in Familial Hypercholesterolemic Swine Implications for Elucidating Disease Etiology - January 26, 2016.… continue reading »


Read more

Jun 9, 2016. Abstract A new technique of elucidating -decay schemes of isotopes with large density of states at low excitation energies has been developed.… continue reading »


Read more

Feb 6, 2017. Elucidation of drug resistance mechanisms entails i recapitulating in vivo tumour behaviour using cell lines derived from primary tumour;.… continue reading »


Read more

There are several instances of co-investigation and related discoveries and achievements in bovine and human immunology; perhaps most interesting is the.… continue reading »


Read more