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During the first step of RDD, complete household census information was obtained for 94% and 81% of the residential telephone numbers contacted for Study I and Study II, respectively.A total of 2,448 men were identified who met the eligibility criteria and 1,645 (71.7%) completed a study interview.The main reasons for non-participation included refusal (29.1%) or too ill to participate (1.4%).Blood samples were drawn and DNA prepared from 1,352 (82.2%) interviewed controls using standard protocols.expression is elevated in tumor epithelial cells as compared to normal epithelium (13,16) and cells expressing the Gly allele of rs351855 grow in tighter colonies, show a slower closure rate in wound assays, and are less invasive in Matrigel than Arg expressing cells (16).In spite of the numerous genetic studies analyzing associations between variants in and cancer, only one non-synonymous SNP (rs351855) has been genotyped in these studies.The fibroblast growth factor receptor 4 (FGFR4) is thought to be involved in many critical cellular processes and has been associated with prostate cancer risk.Four single nucleotide polymorphisms (SNPs) within or near FGFR4 were analyzed in a population-based study of 1458 prostate cancer patients and 1352 age-matched controls.

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The Applied Biosystems (ABI) SNPlex™ Genotyping System was used to genotype the SNPs and proprietary Gene Mapper® software was used for allele calling (In Study II, cases were diagnosed between January 1, 2002, and December 31, 2005 and were 35–74 years of age at diagnosis.Overall, 2,244 eligible prostate cancer patients were identified and 1,754 (78.2%) were interviewed.Discrimination of the specific SNP allele was carried out on the ABI 3730 DNA Analyzer and is based on the presence of a unique sequence assigned to the original allele-specific oligonucleotide.

Quality control included genotyping of 140 blind duplicate samples distributed across all genotyping batches.

Incident cases with histologically confirmed prostate cancer were ascertained from the Seattle-Puget Sound SEER cancer registry.